Uncertainty Evaluation for the Determination of Dexamethasone in Cosmetics by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
10.3969/j.issn.1008-049X.2018.03.051
- VernacularTitle:超高效液相色谱-串联质谱法测定化妆品中地塞米松含量的不确定度分析
- Author:
Jiamin GAO
1
;
Jin CAO
;
Hong DING
Author Information
1. 中国食品药品检定研究院 北京100050
- Keywords:
Dexamethasone;
Uncertainty;
Cosmetics;
UPLC-MS/MS
- From:
China Pharmacist
2018;21(3):539-542,546
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the measurement uncertainty in the determination of dexamethasone in cosmetics by ultra per-formance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Methods: The uncertainty caused by various factors in the whole determination process was analyzed,including weighing,standard solution preparation,calibration fitting,extraction and de-termination. Results:The combined uncertainty in the determination of dexamethasone in cosmetics was 0.75 μg·g-1and the ex-panded uncertainty was 1.5 μg·g-1. The content of dexamethasone in cosmetics was(20.4 ± 1.5) μg·g-1(k=2,confidence inter-val p=95%). Conclusion:The uncertainty of the method is mainly caused by standard solution preparation and calibration fitting.
