Determination of Residual Formaldehyde in EDTA-2Na by Headspace Gas Chromatography
10.3969/j.issn.1008-049X.2018.03.048
- VernacularTitle:顶空气相色谱法测定依地酸二钠中甲醛残留量
- Author:
Le HAI
1
;
Haiyan LONG
Author Information
1. 湖南省药品检验研究院 长沙410001
- Keywords:
Headspace gas chromatography;
EDTA-2Na;
Formaldehyde
- From:
China Pharmacist
2018;21(3):530-532
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a headspace GC method for the determination of residual formaldehyde in EDTA-2Na. Meth-ods:A headspace GC was used to separate the residual solvents on an Agilent DB-WAX (30 m×0.32 mm,0.5 μm) capillary col-umn with an FID detector. The carrier gas was nitrogen at the flow rate of 1.0 ml·min-1. The temperature of the injector was 230 ℃and that of the FID was 260 ℃. The programmed column temperature was set as follows: maintained at 40 ℃ for 6 min, and then raised to 230 ℃ at the rate of 40 ℃·min-1and maintained for 10 min. The injection volume was 1μl with the split ratio of 2:1. The reference solvent and sample were filled into the containers of headspace injector and the containers were in equilibrium at 95℃ for 25 min. The amount of residual formaldehyde was calculated by an external standard method.Results:There was a good linear relationship of formaldehyde reference solvent within the concentration range of 5.10-102.00 μg·ml-1(r=0.999 7). The average recovery was 84.60%(RSD=3.82%,n=9). Conclusion: The method is simple,rapid and accurate for the determination of residual formalde-hyde in EDTA-2Na.
