Simultaneous Determination of Six Effective Components in Dutong Pills by GC
10.3969/j.issn.1008-049X.2018.03.044
- VernacularTitle:GC法同时测定肚痛丸中6种有效成分的含量
- Author:
Xiaolong ZHANG
1
,
2
;
Xiao SUN
;
Chenxu YAO
;
Hongyan CHEN
;
Qinan WU
Author Information
1. 南京中医药大学 南京210023
2. 连云港市食品药品检验检测中心
- Keywords:
Dutong pills;
GC;
Content determination;
Cinnamaldehyde;
Bornyl acetate;
Costunolide;
Dehydrocostus lactone;
Magnolol;
Honokiol
- From:
China Pharmacist
2018;21(3):518-520
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a GC method for the imultaneous determination of cinnamaldehyde, bornyl acetate, costunol-ide,dehydrocostus lactone,magnolol and honokiol in Dutong pills. Methods: The determination was performed on an HP-5 column (30 m ×0.32 mm,0.25 μm) with programmed temperature. The carrier gas was nitrogen with the flow rate of 2.0 ml·min-1. The injection volumn was 1 μl and the sample split ratio was 5:1. The inlet temperature was 280 ℃. The detector was a flame ionization detector with temperature at 300 ℃. Results:The linear ranges were 32.28-516.40 μg·ml-1for cinnamaldehyde(r=0.999 3), 27.06-433.00 μg·ml-1for bornyl acetate(r=0.999 2),25.65-410.40 μg·ml-1for costunolide(r=0.999 3),26.10-417.60 μg ·ml-1for dehydrocostus lactone(r=0.999 3),24.01-384.20 μg·ml-1for magnolol(r=0.999 0) and 18.32-293.10 μg·ml-1 for honokiol(r=0.999 4). The average recovery was 99.71%(RSD=0.67%),99.34%(RSD=1.18%),100.16%(RSD=0. 34%),100.40%(RSD=0.39%),99.32%(RSD=1.22%) and 99.58%(RSD=0.58%)(n=6),respectively. Conclusion:The method is simple,sensitive and accurate,can be used to supplement the insufficient quality control of Dutong pills.
