Simultaneous Determination of Ephedrine Hydrochloride and Amygdalin in Kangzhi Syrup by HPLC
10.3969/j.issn.1008-049X.2018.03.038
- VernacularTitle:HPLC法同时测定抗支糖浆中盐酸麻黄碱和苦杏仁苷的含量
- Author:
Guijun XU
1
;
Wei QI
;
Xuesong WANG
;
Min ZHAO
;
Zhijun LI
;
Youpeng WANG
Author Information
1. 黑龙江中医药大学附属第二医院 哈尔滨150001
- Keywords:
HPLC;
Kangzhi syrup;
Ephedrine hydrochloride;
Amygdalin
- From:
China Pharmacist
2018;21(3):499-501
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC method for the determination of ephedrine hydrochloride and amygdalin in Kangzhi syrup. Methods:The separation was performed on a Waters ODS2(250 mm×4.6 mm,5 μm) column. The mobile phase consisted of acetonitrile-0.2% phosphoric acid solution(containing 0.1% triethylamine) with gradient elution,the detection wavelength was at 205 nm,the flow rate was 1.0 ml·min-1,and the column temperature was 30℃. Results: Ephedrine hydrochloride and amygdalin was linear within the range of 0.169-3.380 μg(r=0.999 9)and 0.141-2.820 μg(r=0.999 7), respectively.The average recovery of e-phedrine hydrochloride and amygdalin was 98.8% and 99.1% with the RSD of 1.93% and 1.22%(n=6), respectively.Conclu-sion:The method is rapid and accurate for the simultaneous analysis of ephedrine hydrochloride and amygdalin, which can provide a reliable way for the quality control for Kangzhi syrup.
