Preparation and Quality Evaluation of Norcantharidin Albumin Nanoparticles
10.3969/j.issn.1008-049X.2018.03.011
- VernacularTitle:去甲斑蝥素白蛋白纳米粒的制备及质量评价
- Author:
Yuye ZHU
1
;
Ling ZHANG
Author Information
1. 咸阳职业技术学院医学院 陕西咸阳712000
- Keywords:
Norcantharidin;
Albumin nanoparticles;
Ultra-high pressure microfluidization technology;
Plackett-Burman experi-mental design;
Box-Behnken experimental design
- From:
China Pharmacist
2018;21(3):420-425
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To prepare norcantharidin albumin nanoparticles and evaluate the physical characteristics of the albumin nanoparticles. Methods:Norcantharidin albumin nanoparticles were prepared by ultra-high pressure microfluidization technology. The average particle size and the drug entrapment efficiency of albumin nanoparticles were used as the evaluation indices. Firstly,Plackett-Burman experimental design was used to screen the formula and process variables which had significant effects on the properties of albu-min nanoparticles,and then Box-Behnken experimental design was used to optimize the variables range. The morphology,particle size distribution,zeta potential and in vitro drug release behavior were investigated. Results:The average particle size of norcantharidin al-bumin nanoparticles was (105.2 ± 30.1) nm,the PdI was 0.127,and the zeta potential was( -24.7 ± 1.9) mV. In 0.5% Tween-80 phosphate buffered saline (pH 7.4),the in vitro cumulative release of norcantharidin albumin nanoparticle suspension reached up to 81.4% in 24 h. Conclusion:The preparation technology of norcantharidin albumin nanoparticles by ultra-high pressure microfluidi-zation technology is simple and feasible. The preparation technology can be used in industrial production.
