Determination of Aflatoxins B1,B2,G1and G2in Xiao'er Fupi Granule by HPLC
- VernacularTitle:HPLC法测定小儿扶脾颗粒中黄曲霉毒素B1、B2、G1、G2含量
- Author:
Xiaoyan HUANG
1
;
Lei FANG
;
Rongwei LI
;
Ye DING
;
Wenli LI
Author Information
1. 湖南省药品检验研究院 长沙410001
- Keywords:
Xiao'er Fupi granule;
Aflatoxin B1;
B2;
G1and G2;
Content determination;
HPLC
- From:
China Pharmacist
2018;21(2):334-336
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for the determination of aflatoxin( AF) B1,B2,G1and G2by HPLC in Xiao'er Fupi granule,and help manufacturers select safe raw materials for production through the analysis of test results of 31 batches of samples and the safety investigation of Xiao'er Fupi granule in terms of aflatoxin pollution. Methods: A post-column photochemical derivation HPLC method was used to detect the content of aflatoxin in Xiao'er Fupi granule. An Ecosil C18column(250 mm×4.6 mm,5 μm) was adopted with the mobile phase of acetonitrile-methanol-water (30: 10: 60)at the flow rate of 0.8 ml·min-1. The column tem-perature was maintained at 30 ℃. The sample size was 20 μl,The excitation wavelength was 360 nm and the emission wavelength was 450 nm in the fluorescence detection. Results:The linear range of aflatoxin B1,B2, G1and G2was 9.65-48.25 pg (r=0.999 1), 2.45-12.25 pg(r=0.999 8), 10.5-52.5 pg(r =0.995 6) and 2.55-12.75 pg (r =0.996 6), respectively. The recovery was 83.3%-95.6%. Totally 14 batches of samples contained aflatoxin,and the total content was 0.21 × 10 -3-0.54 ×10 -3μg·g-1. Conclusion:The method is convenient and accurate,which can be used for the quality control of Xiao'er Fupi granule.
