Batch Inspection of Sildenafil and its Analogues in Anti-fatigue Health Foods by HPLC-MS/MS
10.3969/j.issn.1008-049X.2018.01.039
- VernacularTitle:HPLC-MS/MS用批量检测缓解体力疲劳类保健食品中西地那非及其类似物
- Author:
Yixin ZHOU
1
;
Libo CHEN
;
Bing ZHANG
;
Changping JIA
;
Yefei QIAN
;
Xuan ZHOU
Author Information
1. 苏州市药品检验检测研究中心 江苏苏州215000
- Keywords:
Health foods;
Sildenafil;
Anti-fatigue preparations;
HPLC-MS/MS
- From:
China Pharmacist
2018;21(1):148-150
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To detect 11 kinds of illegally added chemicals in anti-fatigue health foods including sildenafil ,tadalafil, vardenafil , acetildenafil , homosildenafil , hydorxyhomosildenafil , norneosildenafil , aminotadalafil , pseudovardenafil , thioaildenafil and noracetildenafil.Methods:An HPLC-MS/MS method was used with LUNA C18(250 mm ×4.6 mm, 5μm)as the analysis column and triethylamine phosphate , acetonitrile and methanol as the mobile phase with gradient elution at the flow rate of 1ml.min-1 .An ESI+scan and an MS/MS mode were applied .The qualitative and quantitative analysis were carried out by HPLC-MS/MS.Results:Through the detection of 100 batches of samples , 85 batches were only found illegally added chemical substance sildenafil mainly at the concentration of 5-15 mg per granule .Conclusion:The method of HPLC-MS/MS can be used as a powerful screening tool for the rapid drug screening in basic level drug control laboratories to reduce chemical substances illegally added in anti -fatigue health foods .
