Determination of Three Alkaloids in Wine-processed Sophora Flavescens by HPLC
10.3969/j.issn.1008-049X.2018.01.038
- VernacularTitle:HPLC法测定酒炙苦参中三种生物碱的含量
- Author:
Yi XIA
1
;
Fenlan MAO
;
Su XIANG
;
Guoping GAN
Author Information
1. 湖北中医药大学 武汉430065
- Keywords:
Wine-processed Sophora flavescens;
HPLC;
Alkaloids;
Content determination
- From:
China Pharmacist
2018;21(1):146-148
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop an HPLC method for determining matrine , oxymatrine and oxysophocarpine in wine-processed Sophora flavescens.Methods:An HPLC method was used with a Dalian Elite HYP-ODS column (250 mm ×4.6 mm, 5 μm).The mobile phase consisted of methanol-phosphate buffer (12:88) with the flow rate of 1.0 ml· min-1 .The detection wavelength was 205 nm, the column temperature was 25℃and the injection volume was 20 μl.Results:The calibration curve was linear within the range of 0.105-0.420 μg for matrine,0.760-3.040μg for oxysophocarpine and 1.615-6.460μg for oxymatrine.The average recovery of ma-trine, oxysophocarpine and oxymatrine was 99.70％(RSD=1.92％), 99.41％( RSD=1.93％) and 98.60％(RSD=1.79％)(n=6), respectively.Conclusion:The method is accurate and reliable with good reproducibility , promising specificity and high sensitivi-ty, which can be used for the quality control of wine-processed Sophora flavescens.
