Research on HPLC Specific Chromatogram of Xanthii Fructus
10.3969/j.issn.1008-049X.2018.01.017
- VernacularTitle:苍耳子药材的HPLC特征图谱研究
- Author:
Huiming LUO
1
;
Bingyi XIAO
;
Ping NIE
;
Zhimin HE
;
Wenli LI
;
Hui SUN
Author Information
1. 湖南省药品检验研究院 长沙410001
- Keywords:
Xanthii fructus;
HPLC;
Specific chromatogram
- From:
China Pharmacist
2018;21(1):71-74
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the HPLC specific chromatogram and provide comprehensive evaluation of Xanthii fructus from different regions.Methods:The HPLC analysis was performed on an Alltima C18 column (250 mm ×4.6 mm,5μm) with acetonitrile-0.1％phosphoric acid solution as the mobile phase with gradient elution at a flow rate of 0.8 ml· min-1 .The detection wavelength was 278 nm.The column temperature was 25℃.The software"Similarity Evaluation System for Chromatographic Fingerprint of TCMs"was employed to carry out the similarity analysis of the samples from Henan , Jilin, Anhui and the other regions .Results:The specific chromatogram was preliminarily constructed and 5 common peaks with chlorogenic acid as the reference were identified .Conclusion:The method is scientific basis of the quality assessment of Xanthii fructus with convenient and reliable properties .
