Protective Effect of Water-soluble Total Flavonoids from Isodon Lophanthoides var.gerardianus ( Benth.) H.Hara on H2 O2-induced LO2 Cells Damage
10.3969/j.issn.1008-049X.2018.01.012
- VernacularTitle:狭基线纹香茶菜水溶性总黄酮对H2O2致LO2细胞损伤的保护作用
- Author:
Chuanping FENG
1
;
Haixia DING
;
Song HUANG
;
Youling PENG
;
Yingxin LIU
;
Feiyan LI
Author Information
1. 湖南中医药高等专科学校 湖南株洲412012
- Keywords:
Water-soluble total flavonoids from Isodon lophanthoides var.gerardianus ( Benth.) H.Hara;
H2 O2;
LO2 cells dam-age;
ALT;
AST;
MDA;
Protective effect
- From:
China Pharmacist
2018;21(1):53-57
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the protective effect of the water-soluble total flavonoids from Isodon lophanthoides var.gerardia-nus (Benth.) H.Hara on LO2 cells damage.Methods:The cytotoxicity was evaluated by MTT cell viability determination to confirm the concentration range .Hepatocyte damage model was established by H 2 O2 treatment.After the oxidative stress hepatocyte was coin-cubated with WSTF at different concentrations for various times , the protective effect of WSTF on H 2 O2-induced hepatocyte damage was evaluated by MTT cell viability determination and the content determination of ALT , AST and MDA in cell supernatant .The inhibition of WSTF against H 2 O2-induced LO2 cells apoptosis was evaluated by the quantitative determination of Rhodamine 123 fluorescence and intracellular ROS.Results:The LO2 cells injured by 0.3 mmol· L-1 H2 O2 treatment for 4 h were used as the hepatocyte damage model.The concentration range of WSTF was 0.0312-0.125 mg· ml-1.WSTF could inhibit H2O2-induced injury in LO2 cells and obviously reduce ALT, AST and MDA.Moreover, WSTF could reverse mitochondrial membrane potential depolarization and decrease the amount of intracellular ROS .Conclusion:WSTF exhibits notable protective and curative effects on hepatocyte damage in vitro.
