Determination of Total Arsenic and Arsenic Species in Blood of Acute Promyelocytic Leukemia Patients Treated with Arsenous Acid
10.3969/j.issn.1008-049X.2018.01.006
- VernacularTitle:亚砷酸治疗急性早幼粒细胞白血病患者血中总砷及砷形态测定
- Author:
Shuang HU
1
;
Wenjing WANG
;
Meihua GUO
;
Chunlu GAO
;
Xin HAI
Author Information
1. 哈尔滨医科大学附属第一医院药学部 哈尔滨150001
- Keywords:
HPLC;
Hydride generation-atomic fluorescence spectrometry;
Arsenic species;
Acute promyelocytic leukemia;
Plas-ma;
Blood cells
- From:
China Pharmacist
2018;21(1):23-27
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To quantify the plasma concentrations of inorganic arsenic (As(III) and As(V)) and methylated metabo-lites ( MMA and DMA) , and to detect the total amount of arsenic in blood cells and plasma by high performance liquid chromatogra -phy-hydridegeneration-atomic fluorescence spectrometry ( HPLC-HG-AFS) and HG-AFS methods to clarify the arsenic species in acute promyelocytic leukemia (APL) patients.Methods:The blood cells and plasma were digested by the mixture of HNO 3-H2O2 and ana-lyzed by HG-AFS.For the arsenic species , the plasma samples were prepared with perchloric acid to precipitate protein .The superna-tant was separated on an anion-exchange column in 6 min with isocratic elution using 13 mmol · L-1 CH3 COONa, 3 mmol · L-1 NaH2 PO4 , 4 mmol· L-1 KNO3 and 0.2 mmol· L-1 EDTA-2Na.Results:The methods provided linear range of 0.2-20 ng· ml-1 for total arsenic and 2.0-50 ng· ml-1 for four arsenic species (r>0.9950).The spiked recoveries ranged from 81.2％to 108.6％, and the coefficients of variation for intra-and inter-batch precision were less than 9.3％and 12.5％, respectively.The developed methods were applied successfully in the assay of total arsenic and arsenic species in 5 APL patients.Conclusion:The method is simple, fast and accurate , which can be applied in the assay of arsenic compounds in plasma and blood cells in APL patients .
