Optimization of Alcohol Precipitation Technology for Dingtongning Granule Based on Index Components and Pharmacodynamics
- VernacularTitle:基于指标成分和药效学优选定痛宁颗粒的醇沉工艺
- Author:
Lei ZHANG
1
;
Jingtian LIU
Author Information
1. 昆山市中医医院药剂科 江苏昆山215300
- Keywords:
Dingtongning granule;
Alcohol precipitation technology;
Pharmacodynamics;
Orthogonal test;
Comprehensive eval-uation method
- From:
China Pharmacist
2017;20(11):1979-1982
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To optimize the alcohol precipitation technology of Dingtongning granule and investigate the rationality of al-cohol precipitation technology by pharmacodynamics. Methods:With the yield of extract and the transfer rates of paeoniflorin and feru-lic acid as the comprehensive evaluation indices,L9(34) orthogonal test was used to optimize the alcohol precipitation technology by taking the concentration of alcohol precipitation,relative density of extract and alcohol precipitation time as the influencing factors,and the efficacy of water extract before and after the alcohol precipitation was compared through the biochemical indices of mice. Results:The optimum alcohol precipitation process was as follows:the extracting liquid was concentrated to the relative density of 1.10 g·ml-1 (25 ℃),and then 90% ethanol was added till the concentration of ethanol up to 70%,and last,the extract was stored in cold for 18 h. The efficacy had no obvious changes before and after the alcohol precipitation. Conclusion:The optimal alcohol precipitation tech-nology is stable and feasible,which can maintain the efficacy and shows good purification. The optimal alcohol precipitation technology provides experimental basis for the scientific production of Dingtongning granule.
