Effects of Voriconazole Sulfonated Butyl Ether- β-Cyclodextrin Inclusion Compound Eye Drop on Fungal Keratitis in Rabbits
- VernacularTitle:伏立康唑磺丁基醚-β-环糊精包合物滴眼液治疗兔真菌性角膜炎的实验研究
- Author:
Yongjun HU
1
;
Ling SONG
;
Jiji ZHANG
Author Information
1. 湖北省妇幼保健院药剂科 武汉430070
- Keywords:
Voriconazole;
Sulfonated butyl ether-β-cyclodextrin;
Inclusion compound;
Eye drops;
Fungal keratitis;
Fluconazole
- From:
China Pharmacist
2017;20(11):1942-1944
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of voriconazole sulfonated butyl ether-β-cyclodextrin inclusion compound(VCZ-SBE) eye drop on fungal keratitis (FK) in rabbits. Methods:Aspergillus fumigatus was used for the establishment of FK model in rabbits. Totally 48 healthy rabbits were divided into four groups. The 12 right eyes in group A were inoculated with Aspergillus fumigatus with-out any treatment. The 12 right eyes in group B were inoculated with Aspergillus fumigatus,and then treated with 0.5% VCZ-SBE eye drop,those in group C were inoculated with Aspergillus fumigatus,and then treated with 1.0% VCZ-SBE eye drop,and those in group D were inoculated with Aspergillus fumigatus,and then treated with 0.5% fluconazole eye drop. The cornea of normal left eyes in the four groups was used for the blank control. Ulcer grading was performed for all the eyes in 0,1,4,7 and 14 d after the successful es-tablishment of FK model. Every 3 rabbits were sacrificed in 1,4,7 and 14 d,and then the cornea was obtained to evaluate the infiltra-tion degree of inflammatory cells and the invasion depth of hyphae into cornea. Results:After the successful establishment of FK mod-el,the ulcer grading among the groups showed no significant difference(P>0.05). Respectively after the 7,4 and 14-day treatment, the ulcer grading in group B,C and D was notably lower than that in group A (P<0.05). After the 4,7 and 14-day treatment,the ulcer grading,infiltration depth and necrosis degree in group C were all lower than those in group B and D(P<0.05),and after the 7 and 14-day treatment,those in group B were lower than those in group D(P<0.05). Conclusion:Compared with fluconazole eye drop at the same concentration,VCZ-SBE inclusion compound eye drop can decrease the ulcer grading of FK and reduce the infiltration depth in a concentration-dependent manner.
