Determination of Paraquat in Human Serum by HPLC
- VernacularTitle:HPLC法测定人血清中百草枯含量
- Author:
Hua LU
1
;
Daohai CHENG
;
Wenling CHEN
;
Ming SU
;
Zong NING
Author Information
1. 广西医科大学第一附属医院 药学部 南宁 530021
- Keywords:
Paraquat;
Human serum;
HPLC
- From:
China Pharmacist
2017;20(11):1919-1921
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC method for the determination of paraquat in human serum.Methods:The analytical column was a Kromasil C18column (200mm×4.6mm,5μm).The mobile phase was a mixture of acetonitrile and water ( containing 0.03 mol·L-1sodium heptanesulfonate and 0.24 mol·L-1phosphoric acid) (3 :97,pH was adjusted to 2.0 by triethylamine),the detection wavelength was set at 258 nm,the column temperature was 25℃,the injection volume was 20 μl,and the flow rate was 0.8 ml·min-1.Results:The calibration curve of paraquat was linear within the range of 0.106-10.6 mg·L-1( r =0.999 3),and the lower limit of detection was 0.065 mg·L-1. The absolute recovery of paraquat at low,medium and high concentration was more than 89.4%,and the method recovery was more than 94.4%. The intra-day RSDs were 0.12%-1.74%,and the inter-day RSDs were 0.44%-2.89%.Conclusion:The method is simple,quick,accurate,sensitive and specific,and can be used for detecting paraquat con-centration in human serum.
