Angiogenesis function of astragaloside IV in rats with myocardial infarction via PKD1-HDAC5-VEGF pathway
- Author:
Lei YANG
1
;
Nuan LIU
;
Wei ZHAO
;
Xing LI
;
Qian WANG
;
Li HAN
;
Wen-Jing QI
;
Yan-Ke WANG
;
Bing-Yu MAO
Author Information
1. Henan Key Laboratory of Zhang ZhongJing Formulae and Herbs for Immunoregulation
- Keywords:
astragaloside IV;
protein kinase D1;
vascular endothelial growth factor;
myocardial infarction;
class II histone deacetylase 5;
angiogenesis
- From:
Chinese Journal of Pharmacology and Toxicology
2018;32(4):323-324
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE This study aimed to investigate the role and mechanism of Astragaloside IV (AS-IV)in rats with myocardial infarction.METHODS The myocardial infarction model was established by ligation of the left anterior descending artery. The rats were randomly divided into sham, DMSO, model group, AS-IV and CID755673 groups. The rats were sacrificed 4 weeks later, and segmental heart samples were used for hematoxylin and eosin staining and masson staining. The expression of PKD1, HDAC5 and VEGF were analyzed using immunohistochemistry, reverse transcription poly-merase chain reaction and western blot. RESULTS Compared with the sham operation and DMSO groups,morphology of myocardium in model group was disordered,accompanied with necrotic myocar-dial cells and obvious collagen tissues. After treatment with AS-IV, the morphology of myocardium was obviously improved, and the number of new blood vessels increased significantly. However, after treatment with CID755673, the myocardial tissue of rats became disordered again, the necrotic cells increased, and some vessels closed. The expression levels of PKD1, HDAC5 and VEGF mRNA and protein in myocardial tissue of model group were significantly lower than the other four groups(P<0.05), whereas these levels in the AS-IV group were significantly higher than those in the other four groups (P<0.01). Additionally, the CID755673 group had significantly higher levels of PKD1, HDAC5 and VEGF mRNA and protein than the sham group, DMSO group and model group (P<0.05). CONCLUSION AS-IV may partly promote the angiogenesis of myocardial tissue in rats with myocardial infarction via the PKD1-HDAC5-VEGF pathway.
