A method to improve success rate of mice melanoma metastasis model in vivo
10.3969/j.issn.1001-1978.2018.09.026
- VernacularTitle:一种提高小鼠黑色素瘤在体转移模型成功率的方法
- Author:
Ze-Hui CHEN
1
;
Liang ZHANG
;
Bing-Yang CHAI
;
Bo ZHANG
Author Information
1. 新疆植物药资源利用教育部重点实验室
- Keywords:
dexamethasone;
melanoma;
melanoma metastasis model;
immunodepression;
C57BL/6 mice;
network pharmacol-ogy
- From:
Chinese Pharmacological Bulletin
2018;34(9):1326-1330
- CountryChina
- Language:Chinese
-
Abstract:
Aim To improve the rate of melanoma metastasis animal model and provide a reliable experimental modeling meth-od for the study of melanoma metastasis mechanism. Methods Five immunosuppressants were selected and then their targets were screened by network pharmacology. Intraperitoneal injection of immunosuppressants and intravenous injection of B16F10 cells were performed on C57BL/6 mice, then the mice were observed, and body weight were recorded daily. The contents of TNF-α, IL-6, IL-10, VEGF and MMP-9 in serum were measured with ELISA kit. The mice were dissected, then the metastasis situa-tion and the number of metastatic nodules in the lung and other organs were evaluated. HE-staining was involved to determine the morphology of metastatic tissues. Results The closeness centrality of cyclosporine and dexamethasone ranked the top and the targets were concentrated in lung and liver. No significant difference in body weight were observed after animal madel in-duced. The contents of TNF-α, IL-6 IL-10, VEGF and MMP-9 in the model group increased. The number of metastatic nodules in the lung significantly augmented with some kind of liver metas-tasis. HE-staining in the lung tissues showed that tumor presen-ted invasive growth. Conclusion By intraperitoneal injection of dexamethasone, the success rate of mice melanoma metastasis model can be greatly increased.
