Effects of curcumin nanoparticles on hyperlipid-induced cardiomyocyte injury
10.3969/j.issn.1001-1978.2018.09.019
- VernacularTitle:姜黄素纳米粒对高脂诱导的心肌细胞损伤的作用
- Author:
Yu ZHOU
1
;
Jing LI
;
Cui-Yu BAO
Author Information
1. 湖北科技学院 药学院
- Keywords:
curcumin nanoparticles;
high fat;
H9c2;
cardiomyocytes;
reactive oxygen;
apoptosis
- From:
Chinese Pharmacological Bulletin
2018;34(9):1283-1288
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the protective effect of curcumin nanoparticles ( Cur-NPs) against high-fat-in-duced cardiomyocyte injury. Methods H9c2 cardio-myocytes were stimulated with palmitic acid ( PA) to establish a rat model of lipotoxicity injury. The Cur-NPs were pretreated. MTT assay was used to detect cell proliferation. The reactive oxygen species ( ROS) kit was used to detect intracellular reactive oxygen spe-cies and the cells were detected with the TUNEL kit. Apoptosis was detected by Western blot, and the ex-pression levesl of endoplasmic reticulum stress and ap-optotic signaling pathway related proteins were deter-mined. Results High fat might cause the decrease of cell proliferation rate. The level of ROS obviously in-creased, and the pathological changes of cell morphol-ogy were evident. Apoptosis was obviously aggravated. The expression of GRP78, CHOP and caspase-3 appar-ently increased, and the Bax/Bcl-2 ratio elevated, which could all be reversed by Cur-NPs. Conclusions Cur-NPs significantly reduces the production of ROS induced by hyperlipidemia and reduces the expression of endoplasmic reticulum stress and apoptosis-related proteins in cardiomyocytes, thereby inhibiting the dam-age of H9c2 cardiomyocytes induced by high fat.
