The study of the protective effect of panoxadiol on Alzheimer’s disease cells based on network pharmacology
10.3969/j.issn.1001-1978.2018.09.018
- VernacularTitle:基于网络药理学的人参二醇对阿尔茨海默病细胞保护作用的研究
- Author:
Xi-Cai LIANG
1
;
Ying-Jia YAO
;
Yu-Ying WANG
;
Xiu-Li LI
;
Ya-Meng WANG
;
Ying LIN
;
Yue SHI
;
Jing-Xian YANG
Author Information
1. 辽宁中医药大学药学院
- Keywords:
Alzheimer’s disease( AD);
network phar-macological analysis;
molecular docking;
PD;
FYN;
Ca2+overload
- From:
Chinese Pharmacological Bulletin
2018;34(9):1275-1282
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the therapeutic effects of main active compounds of panaxadiol ( PD ) in on Alzheimer’s disease ( AD) via network pharmacologi-cal analysis and Mmolecular docking. Methods A to-tal of 107 prescriptions for AD treatment were screened by using network pharmacology, screening for the high-est frequency of ginseng and its target for AD. Use mo-lecular docking technology was used to find components with the highest score for non-receptor tyrosine kinase ( FYN) docking. Then we successfully estimatedestab-lished AD cell model with overexpressinged APP pro-teins in vitro. Next,the cell viability was detected by MTT assay,the cell damage was detected by LDH as-say,the apoptosis and intracellular Ca2+concentration were detected by flow cytometry, and phosphorylated FYN protein expression was detected by Western blot detection of . phosphorylated FYN protein expression. Results Eighteen active components of Gginseng and 29 AD-related targets were screened by the method of network pharmacology. The results of molecular doc-king showed that PD had strong binding effects with FYN. The results showed that PD could increase the survival rate of cells,reduce the release of LDH,reduce apoptosis,and improve AD cells’ intracellular Ca2+o-verload and reduce the expression of FYN-Y416 pro-tein. Conclusion The experimental results of network pharmacology were are verified and the protective effect of PD on AD may be related to inhibition of FYN signa-ling pathway.
