Determination of hainanolidol and its pharmacokinetics in rat plasma by UPLC
10.3969/j.issn.1001-1978.2018.09.016
- VernacularTitle:UPLC法测定大鼠血浆中海南粗榧内酯醇及其药代动力学研究
- Author:
Wei FANG
1
;
Dian-Lei WANG
;
Yan DING
;
Qing-Qing WU
;
Jie WU
;
Zhao-Min YAO
Author Information
1. 安徽中医药大学药学院
- Keywords:
hainanolidol;
UPLC;
rats;
blood concen-tration;
pharmacokinetics;
intravenous injection
- From:
Chinese Pharmacological Bulletin
2018;34(9):1263-1267
- CountryChina
- Language:Chinese
-
Abstract:
Aim To establish a UPLC method for the determination of the concentration of hainanolidol in plasma of rats, and study the pharmacokinetics of hain-anolidol in rat plasma after single dose i. v. administra-tion of hainanolidol (1, 2, 4 mg·kg-1). Methods The UPLC method for the determination of hainanolidol in rat plasma was established using hainanolide as in-ternal standard. The mobile phase was methanol-water (47 ∶ 53), the flow rate was 0.17 mL·min-1, and the detection wavelength was UV 326 nm. The plasma concentration of hainanolidol in rats was determined by UPLC after single-dose intravenous injection in rats with 1, 2 and 4 mg·kg-1of hainanolidol, and the pharmacokinetic parameters were calculated by DAS2.1. Results The result of calibration curve was linear over the range of 0.05 ~10.00 mg·L-1( r = 0.999 6) . The lower limit of quantification was 0.05 mg·L-1. The intra-day and inter-day precision were both lower than 5% , and the extraction recoveries were higher than 85% , respectively. The validated method was successfully applied to the pharmacokinetic study after i. v administration of hainanolidol in rats with do-ses of 1, 2 and 4 mg·kg-1. The T1/2was (39.82 ± 0.92), (40.11 ± 0.79) and (41.61 ± 2.07) min, respectively. The AUC0-twas ( 65.77 ± 1.08 ) , (130.48 ± 1.26) and (268.75 ± 1.24) min·mg· L-1, respectively. Conclusion A simple and specific UPLC method for the analysis of hainanolidol is suc-cessfully developed, which could be applied to phar-macokinetic study in rat plasma.
