Effect of soyasaponin on drug resistance of ovarian cancer cells
10.3969/j.issn.1001-1978.2018.09.13
- VernacularTitle:大豆皂苷对卵巢癌细胞耐药性的影响
- Author:
Chun-Xiao LU
1
;
Yue CUI
;
Dong-Xu HE
;
Xin MA
Author Information
1. 江南大学药学院和医学院
- Keywords:
soyasaponins;
ovarian neoplasms;
drug resistance;
EMT;
N-cadherin;
E-cadherin;
cell migra-tion
- From:
Chinese Pharmacological Bulletin
2018;34(9):1249-1253
- CountryChina
- Language:Chinese
-
Abstract:
Aim To screen the most effective soyasa-ponins for reversing the drug resistance of ovarian cancer cells, and investigate the possible mechanism. Methods Cell viability assay was used to analyze the drug resistance of A2780/PTX cells treated with four different common soyasaponins respectively,in order to screen out the most effective soyasaponin. Then, the most effective soyasaponin was used to detect the ex-pression of epithelial-mesenchymal transition ( EMT)-related marker proteins, including N-cadherin and E-cadherin,with Western blot and confocal microsco-py. Finally, transwell assay and wound healing assay were applied to observe effect of soyasaponin on regula-ting cancer cell migration. Results Compared with other soyasaponins,soyasaponin Ac most effectively re-versed the drug resistance of A2780/PTX cells. The expression of N-cadherin decreased while that of E-cadherin increased in A2780/PTX cells when treated with soyasaponin Ac for 48 h. The results of transwell and wound healing assay suggested that soyasaponin Ac also reduced the migration of A2780/PTX cells. Con-clusion Soyasaponin Ac can decrease the drug resist-ance via EMT pathway and weaken the migration abili-ty of ovarian cancer cells.
