Sodium aescinate inhibits proliferation of hepatocellular carcinoma through CARMA3/NF-κB signaling pathway
10.3969/j.issn.1001-1978.2018.09.012
- VernacularTitle:七叶皂苷钠通过CARMA3/NF-κB信号通路抑制肝癌细胞的增殖
- Author:
Wei-Xiang LI
1
;
Da-Chen ZHOU
;
Shi WANG
;
Xiao CUI
;
Hui HOU
;
Xiao-Ping GENG
Author Information
1. 安徽医科大学第二附属医院肝胆外科
- Keywords:
CARMA3;
NF-κB;
sodium aescinate;
hepatocellular carcinoma;
immunohistochemistry;
sig-naling pathway
- From:
Chinese Pharmacological Bulletin
2018;34(9):1243-1248
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the expression of CAR-MA3, NF-κB in hepatocellular carcinoma tissues and the underlying mechanism of sodium aescinate in inhib-iting the proliferation of human hepatocellular carcino-ma cells. Methods The expression of CARMA3 and NF-κB in HCC tissues were detected by tissue microar-ray immunohistochemistry. MTT was used to determine the effect of sodium aescinate on the proliferation of HCC cells. Cell apoptosis was detected by flow cytom-etry. The expression of CARMA3, NF-κB protein in HepG2 and Hep3B cells treated with sodium aescinate was detected by Western blot and cell immunofluores-cence. Results Tissue microarray analysis showed that the expression of CARMA3 in HCC was up-regulated compared with the adjacent adjacent liver tissues, and the histopathological differentiation, TNM stage, tumor volume and prognosis were correlated. Sodium aesci-nate in 40 μmol·L-1concentration ( IC50) inhibited the growth of HCC cell lines, promoting its apoptosis, but without toxic effects on normal liver cells. Western blot and cell immunofluorescence detection of sodium aescinate could significantly inhibit the expression of CARMA3 and NF-κB. Conclusion Sodium aescinate can effectively inhibit the proliferation of HCC cells by inhibiting the activation of CARMA3/NF-κB signaling in HCC.
