Effect of rhynchophylline on expression of TH in methamphetamine-induced CPP mice
10.3969/j.issn.1001-1978.2018.09.009
- VernacularTitle:酪氨酸羟化酶与钩藤碱抑制甲基苯丙胺依赖小鼠条件性位置偏爱的关系研究
- Author:
Chen ZHU
1
;
Wei LIU
;
Jing LI
;
Chan LI
;
Miao FANG
;
Zhi-Jie CHEN
;
Yu-Ting ZHOU
;
Chao-Hua LUO
;
Zhi-Xian MO
Author Information
1. 南方医科大学中医药学院中药药理学教研室
- Keywords:
rhynchophylline;
methamphetamine;
con-dition place preference;
drug addiction;
tyrosine hydroxylase;
katamine
- From:
Chinese Pharmacological Bulletin
2018;34(9):1226-1230
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the effect of rhynchophyl-line on the expression of tyrosine hydroxylase ( TH) in hippocampus of methamphetamine-induced condition place preference ( CPP) mice. Methods Metham- phetamine was injected intraperitoneally to mice, and the expression of TH was observed by immunohisto-chemistry and Western blot. Results The CPP mouse model was established successfully by methamphet-amine ( 4 mg·kg-1) . Ketamine ( 15 mg·kg-1) , rhynchophylline low dosage group (40 mg·kg-1) and rhynchophylline high dosage group ( 80 mg·kg-1) could remove the effect of methamphetamine on CPP mice. The result of immunohistochemistry showed that methamphetamine ( 4 mg·kg-1) could increase the number of TH positive cells in hippocampus while ket-amine (4 mg·kg-1), rhynchophylline (40, 80 mg· kg-1) group could attenuate the change. Western blot-ting indicated the expression of TH of model group in-creased significantly, whereas ketamine ( 15 mg· kg-1) , rhynchophylline ( 40, 80 mg·kg-1) group presented less expression. Conclusions The CPP in-duced by methamphetamine in mice may be inhibited to some extent by rhynchophylline, and its mechanism may be associated with the expression of TH.
