Effects of Aconitum coreanum polysaccharides on TGF-β induced endothelial-mesenchymal transition and its mechanisms
10.3969/j.issn.1001-1978.2018.07.018
- VernacularTitle:黄花乌头多糖对TGF-β诱导内皮间质转化的保护作用及机制研究
- Author:
Ya-Qing LI
1
;
Yan KE
;
Jia-Ye JIANG
;
Han-Qing LI
;
Jia-Qi LIU
;
Yuan LI
;
Zhong-Yuan GAN
Author Information
1. 上海中医药大学教学实验中心
- Keywords:
Aconitum coreanum polysaccharides;
TGF-β;
human umbilical vein endothelial cells;
endo-thelial-mesenchymal transition;
protective effect;
mechanism
- From:
Chinese Pharmacological Bulletin
2018;34(7):983-987
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the protective effect of Aconitum coreanum polysaccharides ( ACP) on TGF-β induced endothelial-mesenchymal transition ( EndMT) and its underlying mechanism. Methods HUVECs EndMT model was reproduced by 10 μg·L-1 TGF-β cultured for 72 h in vitro. The effect of ACP(50, 100, 200 mg·L-1) at various concentrations on TGF-β in-duced EndMT of HUVECs was explored. CCK8 assay was employed to detect the cell viability of HUVECs stimulated by ACP. Scratch test was used to analyze the migration ability of cells. The expressions of CD31, α-SMA, Smad2/3, p-Smad2/3 were deter-mined by Western blot. Results CCK-8 assay showed that various concentrations of ACP could promote HU-VECs viability obviously by 10 μg·L-1 TGF-β cul- tured for 36 h. Scratch test showed that TGF-β en-hanced transfer ability of HUVECs significantly, while this case was apparently reversed under the costimula-tion of TGF-β and ACP. Western blot showed that CD31 expression decreased significantly, and the ex-pressions of α-SMA, p-Smad2, p-Smad3 increased significantly, and this phenomenon could be regressed with ACP. Conclusion ACP can inhibit EndMT in HUVECs induced by TGF-β clearly, and the mecha-nism is related to down-regulation of p-Smad2, p-Smad3.
