Effects of resveratrol on cell proliferation in glomerular mesangial cells cultured under high glucose by inhibiting SphK1/AP-1 signal pathway
10.3969/j.issn.1001-1978.2018.07.014
- VernacularTitle:白藜芦醇调节SphK1/AP-1信号通路抑制高糖诱导的肾小球系膜细胞增殖
- Author:
Yan-Hui DENG
1
;
Wen-Yan GONG
;
Qiang LI
;
Fei-Zhen WU
;
Ping-Ping DUAN
;
He-Qing HUANG
Author Information
1. 南方医科大学第三附属医院药学部
- Keywords:
resveratrol;
diabetic nephropathy;
cell proliferation;
SphK1/AP-1 signaling pathway;
fibrone-ctin;
transformation growth factor β1
- From:
Chinese Pharmacological Bulletin
2018;34(7):959-963
- CountryChina
- Language:Chinese
-
Abstract:
Aim To observe the expression of FN and TGF-β1 in the glomerular mesangial cells induced by high-glucose after the intervention of resveratrol, and further discuss its influence on SphK1/AP-1 signaling pathway. Methods The rat glomerular mesangial cells induced by high glucose were used to observe the effects of resveratrol on cell proliferation after interven-tion. The survival vitality and proliferation of glomeru-lar mesangial cells were determined by MTT, and then FN, TGF-β1 and SphK1 protein expression were deter-mined by Western blot. Also, AP-1 activity was deter- mined by EMSA assay. Results Resveratrol could obviously inhibit the proliferation of high glucose-in-duced glomerular mesangial cells, lower SphK1 expres-sion, inhibit AP-1 activity and thus inhibit the expres-sion of FN, TGF-β1. Conclusions Resveratrol inhib-its the proliferation of high glucose-induced glomerular mesangial cells, which may be closely related to the in-hibition of SphK1/AP-1 signaling pathway.
