Effects of bisbenzylisoquinoline alkaloid tetrandrine derivative HL-27 on biological properties of BLM helicase
10.3969/j.issn.1001-1978.2018.07.010
- VernacularTitle:汉防己甲素衍生物HL-27对BLM解旋酶生物学特性的影响
- Author:
Wang-Ming ZHANG
1
;
Zhang-Wen GE
;
Wen-Tao YAN
;
Wei-Dong PAN
;
Yan-Chao JIAO
;
Jie-Lin LIU
Author Information
1. 贵阳中医学院第一附属医院检验科
- Keywords:
BLM642-1290 helicase;
HL-27;
fluorescence polarization;
binding activity;
unwinding activity;
AT-Pase activity
- From:
Chinese Pharmacological Bulletin
2018;34(7):934-939
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of bisbenzyl-isoquinoline alkaloid tetrandrine derivative HL-27 on the biological properties of the BLM642-1290 helicase. Methods Fluorescence polarization technique was used to investigate the effects of bisbenzylisoquinoline alkaloid tetrandrine derivative HL-27 on the DNA bind-ing activity and unwinding activity of the BLM642-1290 helicase. Malachite green-phosphate ammonium molyb-date colorimetry was used to investigate the effects of HL-27 on the ATPase activity of the BLM642-1290 heli-case. Ultraviolet spectral scanning was used to investi-gate the effects of HL-27 on the conformation of the BLM642-1290 helicase. Results When the concentra-tion of HL-27 reached 33.34 μmol·L-1, the inhibi-tion ratio of dsDNA and ssDNA binding activity of the BLM642-1290 helicase was 41.35% and 59.54% , re-spectively. When the concentration of HL-27 reached 50 μmol·L-1, the inhibition ratio of DNA unwinding activity of the BLM642-1290 helicase was 78.68% . When the concentration of HL-27 reached 100 μmol· L-1, the inhibition ratio of ATPase activity of the BLM642-1290 helicase was 43.8% . Conclusion The DNA binding activity, ATPase activity and unwinding activity of the BLM642-1290 helicase can be inhibited by bisbenzylisoquinoline alkaloid tetrandrine derivative HL-27.
