Nested-PCR and a New ELISA-Based NovaLisa Test Kit for Malaria Diagnosis in an Endemic Area of Thailand.
10.3347/kjp.2014.52.4.377
- Author:
Pimwan THONGDEE
1
;
Wanna CHAIJAROENKUL
;
Jiraporn KUESAP
;
Kesara NA-BANGCHANG
Author Information
1. Graduate Program in Bioclinical Sciences, Chulabhorn International College of Medicine, Thammasat University, Pathumthani, Thailand. kesaratmu@yahoo.com
- Publication Type:Original Article ; Comparative Study ; Evaluation Studies ; Research Support, Non-U.S. Gov't
- Keywords:
Plasmodium falciparum;
Plasmodium vivax;
nested-PCR;
ELISA-based NovaLisa test kit
- MeSH:
Adolescent;
Adult;
Aged;
Aged, 80 and over;
Coinfection/*diagnosis/epidemiology;
Endemic Diseases;
Enzyme-Linked Immunosorbent Assay/methods;
Female;
Humans;
Malaria, Falciparum/*diagnosis/epidemiology;
Malaria, Vivax/*diagnosis/epidemiology;
Male;
Middle Aged;
Molecular Diagnostic Techniques/*methods;
Polymerase Chain Reaction/*methods;
Sensitivity and Specificity;
Serologic Tests/methods;
Thailand/epidemiology;
Young Adult
- From:The Korean Journal of Parasitology
2014;52(4):377-381
- CountryRepublic of Korea
- Language:English
-
Abstract:
Microscopy is considered as the gold standard for malaria diagnosis although its wide application is limited by the requirement of highly experienced microscopists. PCR and serological tests provide efficient diagnostic performance and have been applied for malaria diagnosis and research. The aim of this study was to investigate the diagnostic performance of nested PCR and a recently developed an ELISA-based new rapid diagnosis test (RDT), NovaLisa test kit, for diagnosis of malaria infection, using microscopic method as the gold standard. The performance of nested-PCR as a malaria diagnostic tool is excellent with respect to its high accuracy, sensitivity, specificity, and ability to discriminate Plasmodium species. The sensitivity and specificity of nested-PCR compared with the microscopic method for detection of Plasmodium falciparum, Plasmodium vivax, and P. falciparum/P. vivax mixed infection were 71.4 vs 100%, 100 vs 98.7%, and 100 vs 95.0%, respectively. The sensitivity and specificity of the ELISA-based NovaLisa test kit compared with the microscopic method for detection of Plasmodium genus were 89.0 vs 91.6%, respectively. NovaLisa test kit provided comparable diagnostic performance. Its relatively low cost, simplicity, and rapidity enables large scale field application.
