The protective effect of QZXK in nerve injury using zebrafish model induced by MPTP
10.3969/j.issn.1001-1978.2018.06.026
- VernacularTitle:芪智消渴颗粒对MPTP诱导斑马鱼神经损伤的保护作用
- Author:
Rong-Chun WANG
1
;
Li-Fang CHENG
;
Li-Wen HAN
;
Qiu-Xia HE
;
Xi-Qiang CHEN
;
Hai-Rong HOU
;
Xin-Jun ZHANG
;
Ying LI
;
Ke-Chun LIU
Author Information
1. 齐鲁工业大学(山东省科学院)
- Keywords:
QZXK;
zebrafish;
MPTP;
PC12 cells;
nerve protection;
apoptosis
- From:
Chinese Pharmacological Bulletin
2018;34(6):873-878
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the protective effect of Qi ZhiXiaoke granules ( QZXK ) on nerve injury using zebrafish and nerve cell injury models. Methods The nerve injury model was established using wild zebrafish AB line, 72 hours after fertilization treated with 1-methyl-4-phenyl-1 , 2 , 3 , 6-four pyridine ( MPTP ) .Then QZXK of different doses were administered for three days,and the trajectory of the zebrafish behavior was recorded and analyzed. Neuroblastoma PC12 cells were incubated with different concentrations of QZXK and MPTP,and the cell viability of PC12 cells was de-tected by MTT. The mitochondrial membrane potential and expression of apoptosis related protein Caspase3 were measured by kits. Results Compared with con-trol group,MPTP reduced the movement distance of ze-brafish,and with the increase of concentration, QZXK promoted the movement distance and reversed the swimming behavior abnormality of zebrafish. Compared with control group, QZXK could inhibit the apoptosis induced by MPTP and promote the cell viability of PC12 cells with MPTP. QZXK improved the membranepotential and decreased the expression of Caspase3 . Conclusions QZXK exerts neuroprotective effect in the process of nerve injury induced by MPTP. The mechanism may be related with inhibiting apoptosis of neural cells. These experiment provides experimental and theoretical foundation for QZXK promoting cogni-tive function.
