Study on antidepressant effect and mechanism of self-made prescription of strengthening spleen and replenishing Qi
10.3969/j.issn.1001-1978.2018.06.025
- VernacularTitle:自拟健脾益气方药的抗抑郁作用及其机制研究
- Author:
Qian NIU
1
;
He-Qin ZHAN
;
Yan JIANG
;
Zheng-Hang ZHAO
;
Hui-Ru LIU
;
Yu HU
Author Information
1. 新乡医学院药学院
- Keywords:
SMP-SSRQ;
depression;
prefrontal cor-tex;
hippocampus;
Igsf11;
Pdia2;
Sec14 l2
- From:
Chinese Pharmacological Bulletin
2018;34(6):867-872
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the antidepressant effect and the mechanism of self-made prescription of strengthening spleen and replenishing Qi ( SMP-SS-RQ) . Methods The depression model of chronic un-predictable mild stress ( CUMS ) was established in mice. The animals were randomly divided into control group, model group, SMP-SSRQ low, middle and high dose groups (8, 16, 32 mg·kg-1), with 12 mice for each group. The control group and the model group were given the same volume of saline, and other groups were given corresponding dose of SMP-SSRQ. Animals of each group were administered by gavage twice a day for two weeks. Behavioral indexes of mice were deter-mined by open field experiment, sugar consumption test, forced swimming test and tail suspension test. Quantitative real-time PCR (qPCR) and Western blot were respectively performed to detect mRNA and pro-tein expression levels of Igsf11 , Pdia2 and Sec14 l2 inprefrontal cortex and hippocampus. Results Com-pared with model group, depressed mice' s horizontal mobile score, upright number and the sugar water pref-erence index increased and FST as well as TST de-creased in all SMP-SSRQ groups(P<0.05). The de-pression symptoms in mice were obviously improved by SMP-SSRQ therapy. Low and high doses of SMP-SSRQ significantly reduced mRNA and protein expression lev-el of Igsf11 , Pdia2 and Sec14 l2 in the prefrontal cortex and hippocampus of the depressed mice, presenting significant statistical difference compared with model group ( P<0.05 ) . Conclusions SMP-SSRQ can ef-fectively improve mouse depressive behavior, and its mechanism may be related to the down-regulation of Igsf11 , Pdia2 and Sec14 l2 in mouse prefrontal cortex and hippocampus.
