Expression and function of Orai1-BK Ca complex in high glucose treatment rat glomerular mesangial cells
10.3969/j.issn.1001-1978.2018.06.021
- VernacularTitle:高糖对肾小球系膜细胞BKCa-Orai1信号复合物表达与功能的影响
- Author:
Juan DU
1
;
De-Jun YAN
;
Yun-Yun YANG
;
Shuo CHEN
;
Yuan WEI
;
Bing SHEN
Author Information
1. 安徽医科大学基础医学院生理学教研室
- Keywords:
glomerular mesangial cells;
high glucose;
BKCa;
Orai1;
SOCE;
membrane potential
- From:
Chinese Pharmacological Bulletin
2018;34(6):846-850
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate whether Orai1 and BKCa form a physical and functional signal complex in glomerular mesangial cells ( GMCs) and reveal the role of high glucose treatment on Orai1-BKCa complex and SOCE-induced hyperpolarization. Methods The in-teraction of Orai1 and BKCa was evaluated by co-immu-noprecipitation ( co-IP ) . Western blot method was used to detect the expression levels of Orai1 and BKCa . SOCE was activated by Ca2+ depletion evoked by TG in normal glucose and high glucose. The DiBAC4(3) was employed as fluorescence indicator to measure the potential change of membrane. . Results The co-IP experiment results showed that Orai1 interacted with BKCa in GMCs. SOCE induced the hyperpolarization of GMCs. SOCE and SOCE-induced membrane hyperpol-orizaiton were enhanced by high glucose treatment for three days. In addition, the expression levels of Orai1 and BKCa were enhanced significantly in the high glu-cose-cultured cells. Conclusions Orai1 can form a signaling complex with BKCa , which participates in the regulation of hyperpolarization in GMCs and may be in-volved in the hyperpolarization in high glucose cultured GMCs.
