Effect of baicalein on pancreas insulin secretion in rats and its mechanism
10.3969/j.issn.1001-1978.2018.06.016
- VernacularTitle:黄芩素对大鼠胰岛素分泌的作用及机制研究
- Author:
Yang-Yan GUO
1
;
Meng-Meng LIU
;
Xiao-Hua YANG
;
Le-Le REN
;
Zhi-Hong LIU
;
Yun-Feng LIU
;
Yi ZHANG
Author Information
1. 山西医科大学基础医学院药理学教研室
- Keywords:
baicalein;
pancreatic islet;
islet β cell;
insulin secretion;
voltage-dependent potassium chan-nels;
calcium imaging
- From:
Chinese Pharmacological Bulletin
2018;34(6):820-824
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of baicalein on insulin secretion from rat islets and the underlying mechanism. Methods Pancreatic islets were obtained from the pancreas of male Wistar rats by collagenase P digestion and histopaque-1077 density gradient separa-tion. Single islet cells were dispersed from pancreatic islets by Dispase II digestion. Insulin secretion experi-ment was applied to observe insulin release after baica-lein stimulation. To study the potential mechanism, calcium imaging technique and patch-clamp experiment were applied to measure intracellular Ca2+ concentra-tion and voltage-dependent potassium channel currents (Kv). Results In 16. 7mmol·L-1 glucose, baica-lein accelerated insulin secretion in a dose-dependent manner. Baicalein promoted the intracellular Ca2+ con-centration. The patch-clamp experiment showed that baicalein inhibited Kv current in a dose-dependent manner. Conclusion Baicalein can increase the in-tracellular Ca2+ concentration by inhibiting Kv chan-nels and eventually promoting insulin secretion.
