Study on absorption activity of five components of Polygonum orientale L. Flower in Caco-2 cell monolayer
10.3969/j.issn.1001-1978.2018.06.007
- VernacularTitle:荭草花中五种成分在Caco-2细胞中的吸收特性研究
- Author:
Hao CHEN
1
;
Xiao-Li QIN
;
Tao XIA
;
Yue-Ting LI
;
Zi-Peng GONG
;
Yong-Jun LI
;
Lin ZHENG
Author Information
1. 贵州医科大学贵州省药物制剂重点实验室/药用植物功效与利用国家重点实验室
- Keywords:
Polygonum orientale L. Flower extract;
Caco-2 cell;
P-glycoprotein inhibitors;
absorption mechanism;
UPLC-MS/MS;
uptake
- From:
Chinese Pharmacological Bulletin
2018;34(6):764-769
- CountryChina
- Language:Chinese
-
Abstract:
Aim To observe the absorption activity of five components of Polygonum orientale L. Flower ex-tract in Caco-2 cell monolayer. Methods The effects of different concentrations, time, temperature, pH and P-glycoprotein inhibitors on Caco-2 cells were investi-gated by UPLC-MS/MS. Results The absorption of five components of Polygonum orientale L. Flower ex-tract presented a concentration-and time-dependent manner, and the uptake of quercetin was reduced in Caco-2 cells after 90 min. There was a highest intake at 37℃,and the uptake of four components was best in acid environment except for the quercetin at pH 6 , which was best at pH5 . The uptake of quercetin and kaempferol was significantly improved after the addition of P-glycoprotein inhibitors of verapamil. Conclusions The cellular uptake mechanisms of the five compo-nents of Polygonum orientale L. Flower extract is man-inly through passive diffusion. P-glycoprotein is in-volved in the uptake of quercetin and kaempferol.
