In vitro study of genistein inducing apoptosis in MDA-MB-231 breast cancer cells via NF-κB and MAPK signaling pathways
10.3969/j.issn.1001-1978.2018.05.020
- VernacularTitle:NF-κB和MAPK信号通路参与金雀异黄酮诱导乳腺癌MDA-MB-231细胞凋亡的体外研究
- Author:
Li-Qun WEI
1
;
Cheng-Fei XU
;
Wan-Ting LI
;
Xiao-Hang PAN
;
Dao-Hang HUANG
;
Jia-Liang GAN
;
Shuang-Yi TANG
Author Information
1. 广西医科大学第一附属医院药学部
- Keywords:
genistein;
breast cancer;
MDA-MB-231 cells;
apoptosis;
NF-κB;
ERK;
JNK
- From:
Chinese Pharmacological Bulletin
2018;34(5):690-694
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the effect of genistein on apoptosis in human breast cancer MDA-MB-231 cells and the underlying mechanisms. Methods MTT as-say was used to observe the inhibitory rate on human breast cancer MDA-MB-231 cells treated with genistein. Colony assay was used to determine the cell colony formation rate on human breast cancer MDA-MB-231 cells treated with genistein. Western blot was used to detect the expression of Bcl-2, Bax, caspase-3,NF-κB, ERK, p-ERK, JNK and p-JNK in human breast cancer MDA-MB-231 cells treated with genistein. Results The results of MTT assay showed that genistein inhibited the viability of breast cancer MDA-MB-231 cells in a time- and concentration-de-pendent manner. Colony assay suggested that genistein had an antiproliferative effect on MDA-MB-231 cells. The expression levels of Bcl-2, NF-κB and p-ERK were significantly down-regulated compared with con-trol(P < 0.01). However, the expression of Bax, caspase-3 and p-JNK was significantly up-regulated(P<0.01). Conclusions Genistein could inhibit the growth of human breast cancer MDA-MB-231 cells and induce apoptosis,and the mechanism may be related to the inhibition of NF-κB, ERK/MAPK signaling path-ways and the activation of JNK/MAPK signaling path-way.
