Alteration of tetraethylammonium-sensitive potassium current in pulmonary artery smooth muscle cells of pulmonary hypertension rats
10.3969/j.issn.1001-1978.2018.05.015
- VernacularTitle:钾通道阻滞剂四乙基铵敏感性钾电流在肺高压大鼠肺动脉平滑肌细胞中的变化
- Author:
Shuang-Shuang ZHANG
1
;
Yun-Ping MU
;
Zhi-Cheng HE
;
Xiao-Xiao YAN
;
Mo-Jun LIN
Author Information
1. 福建医科大学基础医学院生理学与病理生理学系
- Keywords:
TEA;
voltage-depending potassium chan-nel;
pulmonary hypertension;
pulmonary artery smooth muscle cells;
monocrotaline;
chronic hypoxia
- From:
Chinese Pharmacological Bulletin
2018;34(5):664-669
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the alteration of volt-age-depending potassium channel(KV) current in pul-monary arterial smooth muscle cells(PASMCs) of pul-monary hypertension (PH) rats, and the effect of tet-raethylammonium (TEA,a blocker of KV) on potassi-um channel current in different PH models. Methods The whole-cell patch clamp techniques were applied to record the KVcurrents from PASMCs cultured with Ham's F-12 (1% FBS). Furthermore, the effects of TEA on the KVcurrents were examined in different PH models. Results The whole-cell KVcurrents were ob-viously inhibited in PASMCs of chronic hypoxia (CH)and monocrotaline (MCT)-treated rats. TEA signifi-cantly decreased the whole-cell KVcurrents in PASMCs of control and PH rats,and the inhibitory effect of TEA was dramatically reduced in PH group. Conclusions The degree of the voltage-dependent potassium chan-nels opening is significantly inhibited in PASMCs of CH and MCT-treated rats,accordingly,the TEA-sen-sitive KVcurrents obviously decrease.
