Research of differences of miRNAs expression profiles in different metastatic potential HCC cell lines
10.3969/j.issn.1001-1978.2018.05.014
- VernacularTitle:不同转移潜能肝癌细胞株miRNAs表达谱的差异性研究
- Author:
Jia-Ling SUN
1
;
Bin WEN
;
Hai-Tao SUN
;
Guan-Xin CHEN
;
Xue-Mei YANG
;
Wei-Cong CHEN
;
Hai-Yan AN
;
Jie PANG
;
Song-Qi HE
Author Information
1. 南方医科大学中医药学院
- Keywords:
different metastatic potential;
liver cancer cells;
miRNAs;
gene chip;
bioinformatics analysis;
metastasis and invasion
- From:
Chinese Pharmacological Bulletin
2018;34(5):656-663
- CountryChina
- Language:Chinese
-
Abstract:
Aim To screen the differential microRNA (miRNA) expression profiles of different metastatic po-tential liver cancer cell lines,and predict miRNAs-reg-ulated target genes and their functions. Methods To-tal RNA was extracted and the miRNA expression pro-files were obtained by miRNA microarray chip hybrid-ization. The miRNAs whose expression had significant difference were selected by analyzing the miRNA difference expression profiles of the two different meta-static potential liver cancer cell lines, namely MHCC-97H(high-metastasis) and Hep3B(non-metastasis), which were compared with normal hepatocytes L02 re-spectively. Moreover, we analyzed the miRNA differ-ential expression profile between liver cancer cell lines MHCC-97H and Hep3B. The miRNAs were verified by qPCR and target genes were predicted by four softwares (TargetScan, miRanda, miRWalk, miRDB). To un-derstand the biological functions of predicted target genes, bioinformatics analysis was performed. Results The miRNA microarray results showed that the ex-pression of miR-192-5p and miR-215-5p significantly increased in liver cancer cell lines (MHCC-97H, Hep3B) when compared with normal hepatocytes L02, while miR-130a-3p and miR-196a-5p were significantly reduced; compared with Hep3B, the expression of miR-224-5p markedly increased in liver cancer cell line MHCC-97H, while miR-146a-5p, miR-483-3p and miR-200b-3p were significantly reduced. The re-sults of qRT-PCR were consistent with chip results. Conclusion There are differences of miRNA expres-sion profiles in different metastatic potential liver canc-er cell lines MHCC-97H, Hep3B, and they may par-ticipate in regulating the development and invasion of hepatocellular carcinoma.
