Paeoniflorin-6′-O-benzene sulfonate influences expression of CXCL12/CXCR4 in spleen of adjuvant-induced arthritis
10.3969/j.issn.1001-1978.2018.05.011
- VernacularTitle:CXCL12/CXCR4在佐剂性关节炎大鼠脾脏中的表达及芍药苷-6′-O-苯磺酸酯的作用
- Author:
Mei GAO
1
;
Min ZHANG
;
Min SI
;
Jing-Yu CHEN
;
Wei WEI
Author Information
1. 安徽医科大学临床药理研究所
- Keywords:
adjuvant arthritis;
spleen;
CXCL12;
CX-CR4;
paeoniflorin-6′-O-benzene sulfonate;
complete Freund's adjuvant
- From:
Chinese Pharmacological Bulletin
2018;34(5):639-644
- CountryChina
- Language:Chinese
-
Abstract:
Aim To observe the expression of CXCL12 and its receptor CXCR4 in spleen of rats with adjuvant-induced arthritis (AA) and the effects of paeoniflorin-6′-O-benzene sulfonate (CP-25). Methods AA rats were induced using complete Freund's adjuvant and were randomly divided into normal group,AA group, CP-25 group (50 mg·kg-1) and methotrexate group (MTX,0.5 mg·kg-1),which were treated from d 14 to d 28. HE staining was used to assess the pathologi-cal changes of spleen. The expression of CXCL12 and CXCR4 in spleen was detected by ELISA,immunohis-tochemitry and Western blot. Results CP-25 (50 mg ·kg-1)alleviated the pathological changes of spleen and decreased the expression of CXCL12 and CXCR4 in spleen of AA rats. The pathological changes of spleen and the expression of CXCL12/CXCR4 in spleen revealed a positive correlation. Conclusions Increased expression of CXCL12 and its receptor CX-CR4 may be associated with the pathological changes of spleen in AA rats,which plays an important role in the pathogenesis of RA. CP-25 has obvious therapeutic effect on AA rats and its mechanism may be related to the inhibition of the expression of CXCL12 and CXCR4 in the spleen.
