Comparison of different extraction methods of exosome from macrophages
10.3969/j.issn.1001-1978.2018.04.028
- VernacularTitle:巨噬细胞来源的外泌体不同提取方法的比较
- Author:
Piao-Piao WANG
1
;
Hui-Hui WANG
;
Dai-Yin PENG
;
Shan-Tang ZHANG
;
Wei-Dong CHEN
Author Information
1. 安徽中医药大学药学院
- Keywords:
macrophage;
exosomes;
Western blot;
transmission electron microscopy;
nanoparticle tracker;
polyethylene glycol
- From:
Chinese Pharmacological Bulletin
2018;34(4):589-592
- CountryChina
- Language:Chinese
-
Abstract:
Aim To establish the methods for exosomes isolation from the cell culture medium based on the principle of polyethy-lene glycol (PEG) precipitation combined with ultra-high speed centrifugation. Methods Exosomes were extracted by different centrifugation, PEG6000 precipitation combined with ultra-high speed centrifugation and EXO Quick kit,respectively. The mor-phology and particle size of exosomes were analyzed by transmis-sion electron microscopy,NanoSight ZS and Nano-particle track-er(NTA). Western blot assay was used to identify if the extrac-tion were exosomes. Results According to PEG6000 precipita-tion combined with ultra-high speed centrifugation method, the exosomes were successfully isolated from the culture medium. The morphology and particle size of exosomes,the exosomes iso-lated from PEG6000 precipitation combined with ultra-high speed centrifugation were equal to those of the ultra-high speed centrifugation but smaller to EXO Quick kit. What's more, the results of transmission electron microscopy showed that the three methods had typical discoid vesicles. Conclusion The im-provement of the exosome isolation from cell culture medium is convenient,easy and cheap.
