Effects of L-borneol on chloride channel and cell volume in human umbilical vein endothelial cells
10.3969/j.issn.1001-1978.2018.04.022
- VernacularTitle:左旋龙脑对人脐静脉内皮细胞氯通道和细胞容积的影响
- Author:
Xie-Chou LIANG
1
;
Zhan-Ru CHEN
;
Jing-Kui XU
;
Jie-Zhu FENG
;
Jia-Bao WU
;
Jian-Ying XU
;
Hong GAO
;
Qiu-Chan TAN
;
Li-Xin CHEN
;
Li-Wei WANG
Author Information
1. 暨南大学医学院生理学系
- Keywords:
L-borneol;
human umbilical vein endo-thelial cells;
chloride channel;
patch-clamp tech-nique;
cell volume;
ClC-3
- From:
Chinese Pharmacological Bulletin
2018;34(4):550-556
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the effects of L-borneol on the chloride channel and cell volume of human umbili-cal vein endothelial cells (HUVECs). Methods Whole-cell patch-clamp technique was used to record chloride currents. The expression of ClC-3 protein was down-regulated by siRNA interference technique. The cell volume was measured by dynamic image analysis. Results 20 nmol·L-1L-borneol significantly activa-ted chloride current in HUVEC (79.59 ± 4.90) pA/pF, which could be inhibited by chloride channel blockers,NPPB and DIDS. The outward current inhib-itory rate of NPPB was (95.57 ± 2.57)%, while that of DIDS was (97.28 ± 6.36)%. The chloride current activated by L-borneol significantly decreased after the silence of ClC-3 (27.03 ± 3.89) pA/pF. Cell volume was markedly reduced by L-borneol (14.38 ± 1.58)%,which was inhibited after NPPB appliance. Conclusion L-borneol can activate ClC-3 chloride channel in HUVECs, which induces Cl- outflow then cell volume decrease.
