Effect of Danhong injection on AMPK/SREBP-1/ACC pathways of liver in hyperlipidemic rats
10.3969/j.issn.1001-1978.2018.04.018
- VernacularTitle:丹红注射液对高脂血症大鼠肝脏AMPK/SREBP-1/ACC通路的影响
- Author:
Juan CHEN
1
;
Hu-Feng WANG
;
Jun DENG
;
Hui-Fen ZHOU
;
Hai-Tong WAN
;
Jie-Hong YANG
Author Information
1. 浙江中医药大学基础医学院
- Keywords:
Danhong injection;
AMPK/SREBP-1/ACC pathway;
hyperlipidemia;
fatty acid oxidation;
li-polysis;
fat synthesis
- From:
Chinese Pharmacological Bulletin
2018;34(4):528-532
- CountryChina
- Language:Chinese
-
Abstract:
Aim To discuss the effect of Danhong in-jection(DHI) on hyperlipidemia in rats and its possible mechanism. Methods The hyperlipidemia model of rats were induced by high fat diet. The protein expres-sion of adenosine 5'-monophosphate-activated protein kinase(AMPK), p-AMPK, cholesterol-binding ele-ment binding protein (SREBP-1), adenylate-activated protein kinase carboxylasecetyl-CoA(ACC) and p-ACC in liver were detected using Western blot. Results The protein expression levels of AMPK, SREBP-1 and ACC significantly decreased (P<0.05), but the pro-tein expression levels of p-ACC and p-AMPK signifi-cantly increased (P<0.05). Conclusions Danhong injection can reduce the activity of SREBP-1 and ACC by enhancing the activation of AMPK, and effectively reduce the blood lipid level of hyperlipidemic rats by promoting fatty acid oxidation and reducing lipid depo-sition.
