Effects of astragalus polysaccharide on renal TGF-β1 /Smads signaling pathway in rats with diabetes mellitus
10.3969/j.issn.1001-1978.2018.04.015
- VernacularTitle:黄芪多糖对糖尿病大鼠肾脏TGF-β1/Smads信号通路的影响
- Author:
Cheng-De LI
1
;
Yu WANG
;
Jing-Rong QU
;
Xiao-Jun ZHANG
;
Shu-Mei MAO
;
Ke NIE
Author Information
1. 山东中医药大学基础医学院
- Keywords:
astragalus polysaccharide;
diabetes melli-tus;
renal damage;
TGF-β1;
Smads;
MMP;
TIMP
- From:
Chinese Pharmacological Bulletin
2018;34(4):512-516
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of astragalus polysaccharide (APS) on renal TGF-β1/Smads signa-ling pathway in rats with diabetes mellitus(DM). Methods Wistar rats were randomly divided into nor-mal group,DM group,APS low dose (APS-low) group and APS high dose (APS-high) group. Rats in APS-low group and APS-high group respectively received 200 and 400 mg·kg-1·d-1APS for 8 weeks. Con-centrations of fasting blood-glucose(FBG),blood urea nitrogen and creatinine,as well as urinary kidney injury molecule-1 (KIM-1) and osteopontin (OPN) were measured. Levels of TGF-β1,Smad2,phosphorylated Smad2 (p-Smad2),Smad3,phosphorylated Smad3 (p-Smad3),Smad7,matrix metalloproteinase (MMP) 2,MMP-9,tissue inhibitor of matrix metalloproteinases (TIMP)-1 and TIMP-2 were investigated. Results Compared to control group,DM group had higher levels of FBG,blood urea nitrogen,creatinine KIM-1,OPN, TGF-β1,Smad2,p-Smad2,Smad3,p-Smad3,TIMP-1 and TIMP-2,but lower levels of Smad7,MMP-2 and MMP-9. APS significantly decreased the levels of FBG,blood urea nitrogen,creatinine KIM-1 and OPN, as well as inhibited the activity of TGF-β1/Smads sig-naling pathway. Conclusion The renoprotective effects of APS might be associated with the inhibition of TGF-β1/Smads signaling pathway.
