Effects of rice peptide YGEGSSEEG on improving endothelial cell injury induced by tumor necrosis factor
10.3969/j.issn.1001-1978.2018.04.013
- VernacularTitle:大米α-球蛋白肽YGEGSSEEG对TNF-α诱导的血管内皮细胞损伤的影响
- Author:
Zhi-Yuan JU
1
;
Li-Li WANG
;
Li-Ya LIU
;
Xian-Rong ZHOU
;
Su-Mei ZHOU
;
Li-Tao TONG
Author Information
1. 中国农业科学院农产品加工研究所
- Keywords:
rice α-globulin peptide;
HUVECs;
apop-tosis;
adhesion molecules;
NF-κB signaling pathway;
Bcl-2/Bax
- From:
Chinese Pharmacological Bulletin
2018;34(4):501-507
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the peptides and its protection for vascular endothelial cells, derived from the absorbed components of rice α-globulin,which was shown to be effective in anti-atherosclerosis. Methods The amino acid sequence was purified by gel chro-matography and RP-HPLC, and determined by ESI/MS. Then the peptide was chemically synthesized. Hu-man umbilical vein endothelial cell injury model was induced by tumor necrosis factor-α. The cell viability was measured by cell counting kit to screen the appro-priate peptide intervention concentration. The apoptotic rate was detected by flow cytometry. Bcl-2, Bax, p-p38, vascular cell adhesion molecule and the protein expression level of NF-κB signaling pathway were de-tected by Western blot and immunofluorescent stai-ning. Results Apoptosis of HUVECs induced by TNF-α was significantly increased by YGEGSSEEG, which also regulated expression of Bcl-2/Bax proteins and inhibited phosphorylation of p38 protein. Besides, the peptide suppressed the production of VCAM-1, ICAM-1 and activation of NF-κB pathway. While it did not significantly improve the oxidative stress response in HUVECs. Conclusion Peptide YGEGSSEEG pro-tects vascular endothelial cells through suppressing ap-optosis and expression of adhesion molecules.
