Effect of Kangfuxin on ulcerative colitis induced by trinitrobenzene sulfonic acid in rats
10.3969/j.issn.1001-1978.2018.04.012
- VernacularTitle:康复新液缓解三硝基苯磺酸致大鼠溃疡性结肠炎及其机制研究
- Author:
Han-Chao ZHANG
1
,
2
;
Peng-Chuan WANG
;
Heng LIU
;
Fu-Neng GENG
;
Xiu-Ying MA
;
Miao HE
;
Cheng-Gui ZHANG
;
Yue LI
Author Information
1. 云南省昆虫生物医药研发重点实验室
2. 药用特种昆虫开发国家地方联合工程研究中心
- Keywords:
ulcerative colitis;
Kangfuxin liquid;
2;
4;
6-trinitrobenzene sulfonic acid;
mechanism;
sul-fasalazine;
coloclyster
- From:
Chinese Pharmacological Bulletin
2018;34(4):496-501
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the effect of the Kangfuxin liquid on 2,4,6-trinitrobenzene sulfonic acid (TNBS) induced ulcerative colitis in rats, and to explore its mechanism. Methods Male SD rats were divided into normal control, model control, SASP groups, and Kangfuxin low,medium and high dose groups. In ad-dition to the normal control group, other groups were induced ulcerative colitis with TNBS solution. Disease activity index (DAI), organ index, colon mucosa damage index (CMDI) and histopathological score (HS),the expression levels of IL-4, IL-17 in serum, and MPO, EGF, TGF-β1 in colonic mucosa were de-termined. Results The DAI score showed that the model was successful. Compared with the normal group,the level of IL-4 and IL-17, EGF and TGF-β1 in the model group were reduced significantly, while the CMDI score,HS score,colon index and MPO were elevated significantly. The DAI, CMDI, HS and MPO were reduced (P<0.05 or P<0.01), and the levels of IL-4, IL-17, EGF and TGF-β1 increased signifi-cantly(P <0.01). Conclusions Kangfuxin liquid can effectively alleviate ulcerative colitis induced by TNBS in rats. The mechanism may be related to the down-regulation of MPO expression and up-regulation of IL-4,IL-17,EGF and TGF-β1 levels.
