Correlation between elevated plus and zero mazes as two animal models of state anxiety in Kunming mice
10.3969/j.issn.1001-1978.2018.03.027
- VernacularTitle:高架﹢/〇迷宫实验:经典状态焦虑动物模型相关性研究
- Author:
Shi-Guang SUN
1
;
Xing-Jun HAN
;
Qun WANG
;
Sheng WEI
;
Ming-Qi QIAO
Author Information
1. 山东中医药大学第二附属医院药学部
- Keywords:
elevated plus maze (EPM);
elevated zero maze (EZM);
state anxiety;
animal model;
correlation;
Kunming mice
- From:
Chinese Pharmacological Bulletin
2018;34(3):437-442
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the correlation between elevated plus maze (EPM) and elevated zero maze (EZM),as two type animal models of state anxiety in Kunming mice. Methods The behavior of adult,♂/♀,Kunming mice in EPM and EZM was recorded by sequence,for 5 min,with a one-week inter-trial interval. The following parameters were evaluated:percentage of time in open arms (Otime%) and total entries into open and closed arms(Entries). Subsequently,descriptive analysis,fac-tor analysis,cluster analysis,correlation analysis and consisten-cy test were calculated for these parameters. Results T test showed that, compared with EPM, Otime% of EZM (male/fe-male/both sex) decreased, while Entries (male/both sex) in-creased significantly;Fiedman test showed that Otime% (male/female/both sex) and Entries(female/both sex) of both EPM and EZM had great difference between the 5 repeated fragments;Wilcoxon test showed that, compared with EPM, Otime% of EZM in 1st min (male/female/both sex), 2nd min (male/fe-male/both sex),3rd min (female/both sex) decreased, while the Entries in 1st min (male/both sex), 4th min (male/fe-male/both sex),5th min(both sex) increased markedly. Clus-ter analysis showed that EPM and EZM parameters could be clustered into two groups:EPM group and EZM group(male/fe-male/both sex). Factor analysis indicated that the EPM and EZM parameters could be extracted as two factors: EPM factor and EZM factor (male/female/both sex). Correlation analysis displayed either general(in Otime%) or poor(in Entries) cor-relation(male/female/both sex) between EPM and EZM param-eters. Consistency test displayed general consistency (male/fe-male/both sex) in Otime% between EPM and EZM. Conclu-sions Despite of the similar internal principles to induce state anxiety,the different external environment(structure) results in different behavioral patterns,different structure dimensions,fair correlation and concordance between EPM and EZM, and the stable index of EPM and EZM might be preferred to Otime%.
