Model establishment of skin damage and molecular mechanism on transgenic zebrafish embryos
10.3969/j.issn.1001-1978.2018.03.026
- VernacularTitle:斑马鱼皮肤损伤模型的建立及分子机制
- Author:
Xi-Qiang CHEN
1
;
Rong-Chun WANG
;
Xi-Min WANG
;
Ke-Chun LIU
;
Chong-De XIAO
;
Li-Wen HAN
;
Hai-Rong HOU
Author Information
1. 齐鲁工业大学(山东省科学院)
- Keywords:
vincristine;
zebrafish;
aapoptosis;
caspase-3;
p53;
KRT4
- From:
Chinese Pharmacological Bulletin
2018;34(3):433-437
- CountryChina
- Language:Chinese
-
Abstract:
Aim To establish a new model of skin damage by u-sing vincristine to transgenic zebrafish (krt4:NTR-hKikGR).Methods Skin fluorescent transgenic zebrafish embryos after 24 h fertilization were treated with the 0.01~0.04 mmol·L-1 vincristine,and zebrafish skin cell ablation was investigated un-der fluorescence microscope after 24 h,at same time skin death cells were detected with TUNEL assay and image processed, then the protein expressions of KRT4, caspase-3 and p53 were assessed with Western blot. Results 0.02 mmol·L-1and 0.04 mmol·L-1vincristine could obviously induce zebrafish skin cell apoptosis(P<0.01) with statistically significant differ-ence compared with the control, and the same result could be accomplished in TUNEL assay. Results of Western bolt showed that vincristine could increase the embryos caspase-3 and p53 expression(P <0.01), while vincristine in high concentration might decrease KRT4 markedly(P<0.01). Conclusion Vin-cristine can induce damage on zebrafish skin with suppression KTR4,which can be used as a new skin damage model.
