Farrerol inhibits AngII-induced VSMCs proliferation by reducing Cx43 expression
10.3969/j.issn.1001-1978.2018.03.020
- VernacularTitle:杜鹃素通过降低Cx43的表达抑制AngⅡ诱导的VSMCs增殖
- Author:
Kun ZHANG
1
;
Xiao-Jiang QIN
;
Xiao-Min HOU
;
Yan-Ling HU
;
Qing-Shan LI
Author Information
1. 山西医科大学药学院 山西太原 030001
- Keywords:
farrerol;
Cx43;
Ang II;
VSMCs;
prolifer-ation;
siRNA
- From:
Chinese Pharmacological Bulletin
2018;34(3):401-407
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the role of Cx43 in inhibi-tion of AngII-induced vascular smooth muscle cells(VSMCs) proliferation by farrerol. Methods The primary VSMCs were isolated and cultured by direct adherent culture methods. VSMCs were identified by immunohistochemstry. The cells were divided into the following groups:control group,AngII group,AngII+Farrerol group. The cell viability was measured by CCK-8 cell vitality test. The proliferation of VSMCs was measured by the methods of Edu. The cell cycle of VSMCs was detected by flow cytometry. The mRNA levels of Cx43 were measured by Real-time PCR. The protein levels of Cx43 were measured by Western blot. Results 60 μmol·L-1farrerol could significantly de-crease the cell viability and EdU rate of VSMCs in-duced by AngII(P<0.05),which could also prevent the transformation of VSMCs from G0/G1phase to S phase. The results of real-time PCR and Western blot showed that,compared with the model group,Farrerol could significantly reduce the mRNA and protein ex-pression level of Cx43(P <0.01). After the interfer-ence of Cx43 by siRNA, the inhibition of proliferation by farrerol decreased significantly. Conclusion Far-rerol inhibits AngII-induced VSMCs proliferation signif-icantly, which might be associated with reducing the expression of Cx43.
