The protective effect of broneol on LPS induced acute lung injury
10.3969/j.issn.1001-1978.2018.03.018
- VernacularTitle:莰醇对脂多糖诱导急性肺损伤的保护作用研究
- Author:
Xue-Feng WANG
1
;
Xi-Xi CHEN
;
Jin-Yu CUI
;
Si-Yi TU
;
Shun-De SONG
;
Zhe-Wen ZHANG
;
Hui-Fang TANG
Author Information
1. 浙江中医药大学附属第二医院药剂科
- Keywords:
acute lung injury;
broneol;
alveolar mac-rophages;
alveolar epithelial cells;
lipopolysaccharide;
dexamethasone
- From:
Chinese Pharmacological Bulletin
2018;34(3):388-393
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of broneol on acute lung injury(ALI) induced by lipopolysaccharide (LPS). Methods Male C57 mice were randomly di-vided into saline group, model group, broneol group and dexamethasone group, then the ALI mouse model was induced by instilling intratracheally with LPS. The levels of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),interleukin-6(IL-6) and keratinocyte-de-rived cytokine (KC) were measured at 6h, 12h and 24h after instillation of LPS, and the pathological changes of lung were observed. Mice alveolar macro-phages (MHS) and epithelial cells (MLE-12) were stimulated by LPS. After the stimulation of 1h, 3h, 6h,9h, 12h, 24h, the levels of TNF-α and IL-6 in MHS cells and the contents of KC and macrophage in-flammatory protein-2 (MIP-2) in MLE-12 cells were measured. Results Broneol could inhibit the secre-tion of TNF-α,KC and IL-1β;the early effect of bro-neol on IL-6 was not obvious,but the later effect after the treatment of 24 hours was obvious. After LPS instil-lation 6h and 12h,Broneol could significantly improve lung tissue pathological changes. Broneol had no effect on TNF-α secretion of MHS cells, but it obviously af-fected IL-6 secretion in the later stage. In addition, broneol significantly inhibited KC and MIP2 secretion in MLE-12 cells at the later stage of LPS stimulation. Conclusions Broneol can protect LPS-induced acute lung injury. The mechanism may be related to the inhi-bition of the release of inflammatory factors,the activa-tion of inflammatory cells and the aggregation of neutro-phils.
