Effect of ilexonin A on proliferation and migration of bone mesenchymal stem cells in rats
10.3969/j.issn.1001-1978.2018.03.013
- VernacularTitle:毛冬青甲素对大鼠骨髓间充质干细胞增殖及迁移的影响
- Author:
Qing-Shuang LI
1
;
Guan-Yi ZHENG
;
Bi-Qin ZHANG
;
Xiao-Dong CHEN
;
Qiong JIANG
;
Zhen-Zhen LIU
Author Information
1. 福建医科大学附属协和医院中医科
- Keywords:
bone marrow mesenchymal stem cells;
migration;
ilexonin A;
CXCR4;
proliferation;
incuba-tion
- From:
Chinese Pharmacological Bulletin
2018;34(3):358-364
- CountryChina
- Language:Chinese
-
Abstract:
Aim To observe the effect of ilexonin A (IA) on the proliferation of bone marrow mesenchymal stem cells(BMSCs),and to investigate whether IA can promote the migration of BMSCs by up-regulating the expression of CXCR4 in rats. Methods MTT method was used to assay and analyse the proliferation of BM-SCs which were pretreated with different concentrations of IA (3.125,6.25,12.5,25,50,100,200,400, 800 mg·L-1) for 24,48 and 72h,then the best con-centration and the best optimum time were screened. The third generation of BMSCs was exposed to the opti-mal concentration of IA for 48h. The Transwell system was used to carry out the experiment of BMSCs migra-tion. Western blot was used to analyse the expression of CXCR4. Results MTT assay showed that com-pared with control group, the proliferation of BMSCs was significantly reduced in IA 100 ~800 mg·L-1 groups at 24h(P < 0.05); compared with control group, the proliferation of BMSCs significantly de-creased in IA 100~800 mg·L-1groups at 48h(P<0.05),but markedly increased in IA 6.25 and 3.125 mg·L-1groups (P <0.05); compared with control group,the proliferation of BMSCs was significantly re-duced in IA 12.5~800 mg·L-1groups at 72h(P<0.05). The above results indicated that the BMSCs in-cubated with IA 6.25 and 3.125 mg·L-1for 48h were the optimal choice to promote proliferation. The Transwell migration assay showed that incubation with IA 6.25 and 3.125 mg·L-1for 48h could significant-ly increase the migration of BMSCs(P <0.05), and the migration rate was not related with the concentra-tion of IA. This effect was completely blocked by AMD3100(the antagonist of CXCR4). Western blot showed that incubation with IA 6.25 and 3.125 mg· L-1for 48h could increase the expression of CXCR4 in BMSCs(P<0.05). Conclusion IA can promote the proliferation of BMSCs and increase the migration of BMSCs by up-regulating the expression of CXCR4.
