Effects of Taoren-Honghua drug pair on degeneration of cervical disc cartilage endplate in rat model of dynamic and static forces imbalance
10.3969/j.issn.1001-1978.2018.02.025
- VernacularTitle:桃仁-红花药对对动静力失衡性大鼠椎间盘软骨终板退变的影响
- Author:
Yi-Ru WANG
1
;
De-Zhi TANG
;
Qian-Qian LIANG
;
Hao XU
;
Yong-Jian ZHAO
;
Wei-Chao ZHENG
Author Information
1. 安徽理工大学医学院
- Keywords:
drug pair;
intervertebral disc;
degeneration;
cartilage endplate;
type Ⅱ collagen;
type Ⅹ collagen
- From:
Chinese Pharmacological Bulletin
2018;34(2):279-283
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of TaorenHonghua drug pair on intervertebral disc degeneration (IVDD) in rats.Methods Fifty healthy Wistar rats were randomly divided into control group,model group,sham group,meloxicam group and Taoren-Honghua drug pair group,with 10 rats in each group.We established dynamic and static forces imbalance of cervical disc degeneration model or sham surgery in rats.12 weeks later,rats were intragastrically administered with meloxicam,Taoren-Honghua drug pair or saline for 30 days.C4/5 and C6/7 discs were harvested from rats.ABOG staining was used for observation of intervertebral disc morphology,real time PCR for mRNA expressions of type Ⅱ collagen (Col Ⅱ) and type Ⅹ collagen (Col Ⅹ),and immunohistochemical staining for Col Ⅱ and Col Ⅹ.Results Compared with model group,Col Ⅱ expression increased,while Col X expression decreased in chondrocyte of intervertebral disc in Taoren-Honghua-treated group(P ＜ 0.01).Conclusion Taoren-Honghua drug pair could delay the degeneration of cartilage endplate in rat intervertebral disc.
