Effect of antioxidant peptide AOP1 on healing repair of skin burns in mice
10.3969/j.issn.1001-1978.2018.02.016
- VernacularTitle:抗氧化肽AOP1对小鼠皮肤烫伤创面愈合修复的影响
- Author:
Di QIN
1
;
Hui-Jie ZHANG
;
Xi-Ying YANG
;
Yan-Tong CHEN
;
Wei-Ping LIN
;
Wen-Hui LI
;
Tong-Yi SUN
;
Yuan-Yuan GAO
Author Information
1. 潍坊医学院生物科学与技术学院
- Keywords:
antioxidant peptide;
AOP1;
skin burns;
wound repair;
oxidative stress;
inflammation
- From:
Chinese Pharmacological Bulletin
2018;34(2):225-231
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the effect and mechanism of antioxidant peptide AOP1 on repair of skin burn wound healing in mice.Methods Fluorescence probe DCFH-DA was used to detect the changes of intracellular reactive oxygen species (ROS).Cell proliferation and migration assay were used to detect AOP1 toxicity and its effect on wound healing.Moreover,the skin scald wound was made on the shaved dorsum of the anesthetized mice with a 1.0 cm diameter brass cylinder heated in a water bath at 80 ℃ for 2 min and pressed against the rat skin for 10 s.The effects of AOP1 on the healing of skin burns were observed by HE and Masson staining and the contents of MDA and the activity of SOD in skin tissues were measured.Results The antioxidant peptide AOP1 could significantly reduce the number of ROS in HaCaT and L929 cells,and promote cell migration and proliferation.Compared with the untreated group,the skin healing time of AOP1 group was short,the healing rate was high,the area of scab was small,and inflammation and the content of MDA in burned tissue significantly decreased.The effect of AOP1 on healing of burn wound healing was also confirmed by HE and Masson staining.Conclusion It is suggested that the antioxidant peptide AOP1 with natural activity may promote the healing of skin burns by reducing the oxidative stress caused by burns.
