Formyl peptide receptor-2 enhances inflammatory response induced by lipopolysaccharide in BV-2 cells
10.3969/j.issn.1001-1978.2018.02.012
- VernacularTitle:甲酰肽受体-2促进LPS诱导的BV-2细胞炎症反应
- Author:
Qing WANG
1
;
Li-Juan SONG
;
Jie-Zhong YU
;
Zhi-Chao YANG
;
Wei-Jia JIANG
;
Yan-Hua LI
;
Wen-Juan GUO
;
Cun-Gen MA
Author Information
1. 山西中医药大学国家中医药管理局益气活血重点研究室
- Keywords:
lipopolysaccharide;
BV-2 cells;
FPR2;
microglia;
inflammatory factor;
chemotaxis
- From:
Chinese Pharmacological Bulletin
2018;34(2):202-207
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the expression of formyl peptide receptor-2 (FPR2) in lipopolysaccharide (LPS)-induced-BV-2 cells,and detect FPR2's influence on inflammatory response induced by LPS.Methods After 1 mg · L-1 LPS acting on BV-2 cells at 12 h,the extrinsic inflammatory model was established.We used the Western blot assay to test the levels of FPR2 protein.And the expressions of phosphorylated NF-κB,TNF-α and IL-1β were investigated when the LPS-induced-BV-2 was incubated with FPR2's agonist MMK-1 and antagonist Boc-2.Transwell assay was also used to detect the LPS-inducedBV-2 migration induced by MMK-1 and Boc-2.Resuits LPS up-regulated the expression of FPR2,and when its agonist was acted on LPS-induced-BV-2,the levels of phosphorylated NF-κB,TNF-α and IL-1β were significantly higher than those of LPS group.In addition,the chemotaxis of LPS-induced-BV-2 also increased by MMK-1.These effects were abolished by Boc-2.Conclusions LPS can increase the expression of FPR2 on BV-2 cells,and FPR2 enhances the inflammatory response induced by LPS.
