Bigelovii A induced autophagy in MCF7 cells by inhibiting mTOR signal pathway
10.3969/j.issn.1001-1978.2018.02.011
- VernacularTitle:海蓬子皂苷甲通过mTOR信号通路诱导MCF7细胞发生自噬
- Author:
Fu-Qin GUAN
1
;
Yu SHAN
;
Zhen-Zhen HUANG
;
Qi-Zhi WANG
;
Yu CHEN
;
Min YIN
;
Fei LIU
;
Shu XU
;
Ming WANG
;
You-Yi ZHAO
;
Xu FENG
Author Information
1. 江苏省中国科学院植物研究所
- Keywords:
Bigelovii A;
MCF7 cells;
autophagy;
mTOR;
Akt;
ERK
- From:
Chinese Pharmacological Bulletin
2018;34(2):197-201
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of Bigelovii A on autophagy and its mechanism.Methods Fluorescence microscope,flow cytometry and Western blot were employed to analyze autophagy.Western blot was used to detect the protein expressions of mTOR pathway.MTT colorimetry was used to assay cell viability after treatment with 3-MA and Bigelovii A or Bigelovii A alone.Results Bigelovii A-treated MCF7 cells displayed a dramatic increase in the number of MDC-labeled vesicles and the expressions of LC3-Ⅱ,indicating cell autophagy.Ⅰt was proved that in MCF7 cells,Bigelovii A inhibited mTOR signaling by decreasing Akt and p-ERK.Consistently,Bigelovii A decreased phosphorylation levels of mTOR,p70S6K (Ser371,Thr389) and 4EBP1 proteins.Inhibiting Bigelovii Ainduced autophagy with the autophagy inhibitor 3-methyladenine significantly decreased cell viability,which suggested that Bigelovii A-induced autophagy played a pro-survival role.Conclusion Bigelovii A is likely to induce autophagy through inhibiting mTOR pathway.
